Part:BBa_K1059002:Design

GFP-LVA under B0035 control

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 667
  

Design Notes

The GFP-LVA is from part K145280, but it is reported that the RBS is so weak that it cannot work well, so we have to change the RBS to B0035 by PCR.

Source

The GFP-LVA sequence is from part K145280, we change the RBS B0032 to B0035 and standardize it by PCR .